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Garcia kiko
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Garcia kiko
Introduction, Workflow, and Applications of Sanger Sequencing
What Is Sanger Sequencing? Sanger sequencing, a DNA sequencing method, hinges on the selective incorporation of chain-terminating dideoxynucleotides during the in vitro replication of DNA, guided
February 05, 2025
Garcia kiko
BSA-seq Technology Workflow
Bulked segregant analysis (BSA) is a method first applied in lettuce by R. W. MICHELMORE in 1991 for the rapid localization of genes controlling specific
February 05, 2025
Garcia kiko
Detecting Fusion Transcripts by RNA Sequencing in Screening Tumors
What is fusion gene? What is Chimeric RNAs? A fusion gene is a chimeric gene formed by the fusion of partial sequences of two genes, usually
December 23, 2024
Garcia kiko
Biological Function and Sequencing Technologies of m1A RNA Modification
Introduction to N1-methyladenosine (m1A) Sequencing In eukaryotic cells, N1-methyladenosine (m1A) is found in thousands of different gene transcripts (from yeast to mammals). The majority of m1A
December 23, 2024
Garcia kiko
Overview of Metatranscriptomic Sequencing
What is metatranscriptomic sequencing? Metatranscriptomic sequencing provides direct access to culturable and non-culturable microbial transcriptome information by large-scale, high-throughput sequencing of transcripts from all microbial communities
October 15, 2024
Garcia kiko
Plasmid Detection and Complete Plasmid DNA Sequencing
Introduction to Bacterial Plasmid Bacterial plasmids are circular or linear double-stranded DNA molecules defined by their capability of autonomous replication in the hosts. They are critical
October 15, 2024
Garcia kiko
Viruses and Virome Sequencing
Viruses Viruses, these minuscule and seemingly simple structures, are garnering increasing attention from scientists worldwide. With their unique characteristics and profound impact on living organisms, viruses
August 02, 2024
Garcia kiko
Metagenome Sequencing vs. Viral Amplicon Sequencing: Choosing Effective Sequencing Methods for Monitoring Viral Mutations
The monitoring of viral mutations has gained paramount importance due to the likelihood of mutations occurring during viral transmission. This necessitates a meticulous tracking of
August 02, 2024
Garcia kiko
Unveiling How Microbial Genes Transfer Horizontally by Whole-Genome Sequencing
The majority of the biochemical interaction on the planet is carried out by microbial populations and they perform an essential part in bioprocesses such as
July 29, 2024
Garcia kiko
What Is the Difference Between Aerobes and Anaerobes?
Microorganisms, particularly bacteria, are grouped based on various characteristics such as physical morphology, cultivation characteristics, energy and nutrient requirements, biosynthetic capacities, optimal growth temperature, and
July 29, 2024
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