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Garcia kiko
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Garcia kiko
Principles, Applications, and Optimization in Modern Genomics of GBS vs. RAD-Seq
The technologies of Genotyping by Sequencing (GBS) and Restriction site Associated DNA Sequencing (RAD-Seq) have emerged as dual catalysts in the domain of genetic variation
June 12, 2025
Garcia kiko
Circulating Tumor DNA (ctDNA) vs. Cell-free DNA (cfDNA)
The Difference Between cfDNA and ctDNA Cell-free DNA (cfDNA) refers to the DNA that is liberated into the bloodstream as a result of processes such as
June 12, 2025
Garcia kiko
Exploring the Cell Cycle from A Single-Cell Transcriptome Perspective
Cell division is a fundamental biological process with features and basic mechanisms conserved in all eukaryotes. The process that occurs between the beginning of the
April 09, 2025
Garcia kiko
Mastering Iso-Seq Analysis: From Data Generation to Interpretation
Homologous Sequencing (Iso-Seq) is a single molecule real-time (SMRT) sequencing technology developed by PacBio, which can generate full-length transcript sequences, thus avoiding the complicated transcriptome
April 09, 2025
Garcia kiko
Introduction, Workflow, and Applications of Sanger Sequencing
What Is Sanger Sequencing? Sanger sequencing, a DNA sequencing method, hinges on the selective incorporation of chain-terminating dideoxynucleotides during the in vitro replication of DNA, guided
February 05, 2025
Garcia kiko
BSA-seq Technology Workflow
Bulked segregant analysis (BSA) is a method first applied in lettuce by R. W. MICHELMORE in 1991 for the rapid localization of genes controlling specific
February 05, 2025
Garcia kiko
Detecting Fusion Transcripts by RNA Sequencing in Screening Tumors
What is fusion gene? What is Chimeric RNAs? A fusion gene is a chimeric gene formed by the fusion of partial sequences of two genes, usually
December 23, 2024
Garcia kiko
Biological Function and Sequencing Technologies of m1A RNA Modification
Introduction to N1-methyladenosine (m1A) Sequencing In eukaryotic cells, N1-methyladenosine (m1A) is found in thousands of different gene transcripts (from yeast to mammals). The majority of m1A
December 23, 2024
Garcia kiko
Overview of Metatranscriptomic Sequencing
What is metatranscriptomic sequencing? Metatranscriptomic sequencing provides direct access to culturable and non-culturable microbial transcriptome information by large-scale, high-throughput sequencing of transcripts from all microbial communities
October 15, 2024
Garcia kiko
Plasmid Detection and Complete Plasmid DNA Sequencing
Introduction to Bacterial Plasmid Bacterial plasmids are circular or linear double-stranded DNA molecules defined by their capability of autonomous replication in the hosts. They are critical
October 15, 2024
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